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c1 single cell autoprep ifc microfluidic chip  (fluidigm)


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    Structured Review

    fluidigm c1 single cell autoprep ifc microfluidic chip
    C1 Single Cell Autoprep Ifc Microfluidic Chip, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 621 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c1+single+cell+autoprep+ifc+microfluidic+chip/C1+Single-Cell+Open+App+IFC/pmc07338530-265-10-16
    Average 96 stars, based on 621 article reviews
    c1 single cell autoprep ifc microfluidic chip - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Single Cell:

    Article Title: Quantitative single-cell RNA-seq with unique molecular identifiers.
    Article Snippet: nature methods | ADVANCE ONLINE PUBLICATION | on ES cells, and hypothalamic and cortical primary neurons).. During reverse transcription, each cDNA molecule was tagged with a 5-bp random sequence serving as UMI (Fig. 1a and Supplementary Fig. 2).. We counted cDNA molecules by enumerating the total number of distinct UMIs aligned to each position (Fig. 1b).

    Article Title: Diversity of Interneurons in the Dorsal Striatum Revealed by Single-Cell RNA Sequencing and PatchSeq
    Article Snippet: C1 Suspension Reagent was added (all ‘C1’ reagents were from Fluidigm, Inc.) in a ratio of 4 μL to every 7 μL cell suspension as previously described ( ). .. 11 μL of the cell suspension mix was loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip designed for 10- to 17-μm cells, and the chip was then processed on a Fluidigm C1 instrument using the ‘mRNA Seq: Cell Load (1772x/1773x)’ script (30 min at 4°C). .. The plate was then transferred to an automated microscope (Nikon TE2000E), where a brightfield and RFP or EGFP fluorescence image (20 × magnification) was acquired for each capture site using μManager ( http://micro-manager.org/ (2)), which took < 15 min. Quality of cells and control for doublets was performed after each experiment as described in ( ).

    Article Title: Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development.
    Article Snippet: C1 Suspension Reagent was added (all ’C1’ reagents were from Fluidigm, Inc.) in a ratio of 4mL to every 7mL cell suspension. .. 11mL of the cell suspensionmix was loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip designed for 10- to 17mm cells, and the chip was then processed on a Fluidigm C1 instrument using the ’mRNA Seq: Cell Load (1772x/1773x)’ script (30 min at 4 C). .. The plate was then transferred to an automated microscope (Nikon TE2000E), and a bright-field image (203 magnification) was acquired for each capture site using mManager (http://micro-manager.org/ (2)), which took <15 minutes.

    Article Title: Transcriptomic and anatomic parcellation of 5-HT 3A R expressing cortical interneuron subtypes revealed by single-cell RNA sequencing
    Article Snippet: Four microliters of C1 Suspension Reagent (Fluidigm) was added to 16 μl of FACS isolated Htr3a -GFP+ cells (300–500 cells per μl cell suspension). .. The 20 μl of the cell suspension mix was loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip designed for 5–10 μm cells, and the chip was then processed (C1 system, Fluidigm) following the manufacturer's protocol. ..

    Article Title: Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development
    Article Snippet: C1 Suspension Reagent was added (all 'C1' reagents were from Fluidigm, Inc.) in a ratio of 4μL to every 7μL cell suspension. .. 11μL of the cell suspension mix was loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip designed for 10- to 17μm cells, and the chip was then processed on a Fluidigm C1 instrument using the 'mRNA Seq: Cell Load (1772x/1773x)' script (30 min at 4°C). .. The plate was then transferred to an automated microscope (Nikon TE2000E), and a bright-field image (20× magnification) was acquired for each capture site using μManager ( http://micro-manager.org/ (2)), which took <15 minutes.

    Article Title: Single‐cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential
    Article Snippet: .. The cells were loaded according to manufacturers protocol on C1 Single‐Cell AutoPrep IFC microfluidic chip (for cell size 10–17 μm) and processed on a Fluidigm C1 instrument. ..

    Article Title: Pre-clinical study of induced pluripotent stem cell-derived dopaminergic progenitor cells for Parkinson’s disease
    Article Snippet: C1 Suspension Reagent (Fluidigm) was added to the cell suspension at a ratio of 40%. .. Six microliters of cell suspension mix were loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip (Fluidigm) designed for cells 10–17 μm in size, and the chip was then processed on a Fluidigm C1 instrument using the “STA: Cell Load (1862×)” script. ..

    Article Title: Single-cell transcriptomic profiling of progenitors of the oligodendrocyte lineage reveals transcriptional convergence during development
    Article Snippet: C1 Suspension Reagent was added (all ‘C1’ reagents were from Fluidigm, Inc.) in a ratio of 4 μ L to every 7 μ L cell suspension. .. 11 μ L of the cell suspension mix was loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip designed for 10- to 17 μ m cells, and the chip was then processed on a Fluidigm C1 instrument using the ‘mRNA Seq: Cell Load (1772x/1773x)’ script (30 min at 4°C). .. The plate was then transferred to an automated microscope (Nikon TE2000E), and a bright-field image (20× magnification) was acquired for each capture site using μ Manager ( http://micro-manager.org (2)), which took <15 minutes.

    Suspension:

    Article Title: Diversity of Interneurons in the Dorsal Striatum Revealed by Single-Cell RNA Sequencing and PatchSeq
    Article Snippet: C1 Suspension Reagent was added (all ‘C1’ reagents were from Fluidigm, Inc.) in a ratio of 4 μL to every 7 μL cell suspension as previously described ( ). .. 11 μL of the cell suspension mix was loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip designed for 10- to 17-μm cells, and the chip was then processed on a Fluidigm C1 instrument using the ‘mRNA Seq: Cell Load (1772x/1773x)’ script (30 min at 4°C). .. The plate was then transferred to an automated microscope (Nikon TE2000E), where a brightfield and RFP or EGFP fluorescence image (20 × magnification) was acquired for each capture site using μManager ( http://micro-manager.org/ (2)), which took < 15 min. Quality of cells and control for doublets was performed after each experiment as described in ( ).

    Article Title: Transcriptomic and anatomic parcellation of 5-HT 3A R expressing cortical interneuron subtypes revealed by single-cell RNA sequencing
    Article Snippet: Four microliters of C1 Suspension Reagent (Fluidigm) was added to 16 μl of FACS isolated Htr3a -GFP+ cells (300–500 cells per μl cell suspension). .. The 20 μl of the cell suspension mix was loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip designed for 5–10 μm cells, and the chip was then processed (C1 system, Fluidigm) following the manufacturer's protocol. ..

    Article Title: Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development
    Article Snippet: C1 Suspension Reagent was added (all 'C1' reagents were from Fluidigm, Inc.) in a ratio of 4μL to every 7μL cell suspension. .. 11μL of the cell suspension mix was loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip designed for 10- to 17μm cells, and the chip was then processed on a Fluidigm C1 instrument using the 'mRNA Seq: Cell Load (1772x/1773x)' script (30 min at 4°C). .. The plate was then transferred to an automated microscope (Nikon TE2000E), and a bright-field image (20× magnification) was acquired for each capture site using μManager ( http://micro-manager.org/ (2)), which took <15 minutes.

    Article Title: Pre-clinical study of induced pluripotent stem cell-derived dopaminergic progenitor cells for Parkinson’s disease
    Article Snippet: C1 Suspension Reagent (Fluidigm) was added to the cell suspension at a ratio of 40%. .. Six microliters of cell suspension mix were loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip (Fluidigm) designed for cells 10–17 μm in size, and the chip was then processed on a Fluidigm C1 instrument using the “STA: Cell Load (1862×)” script. ..

    Article Title: Single-cell transcriptomic profiling of progenitors of the oligodendrocyte lineage reveals transcriptional convergence during development
    Article Snippet: C1 Suspension Reagent was added (all ‘C1’ reagents were from Fluidigm, Inc.) in a ratio of 4 μ L to every 7 μ L cell suspension. .. 11 μ L of the cell suspension mix was loaded on a C1 Single-Cell AutoPrep IFC microfluidic chip designed for 10- to 17 μ m cells, and the chip was then processed on a Fluidigm C1 instrument using the ‘mRNA Seq: Cell Load (1772x/1773x)’ script (30 min at 4°C). .. The plate was then transferred to an automated microscope (Nikon TE2000E), and a bright-field image (20× magnification) was acquired for each capture site using μ Manager ( http://micro-manager.org (2)), which took <15 minutes.



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